OPTIZYME™ BstEII (Eco91I), Fisher BioReagents™

Supplier: Thermo Fisher Scientific BP80381

Catalog No. BP8038-1

Quantity

Description

5'...G^G T N A C C...3'
3'...C C A N T G^G...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl)₂ , 100mM NaCl and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with BstEII.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of BstEII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: G^GTSACC - MaeIII, NmuCI; G^GTWACC - MaeIII

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: May overlap - no effect

3'...G GWm5CCANTG GWm5CC...5'

(No cleavage effect)

CpG: May overlap - no effect

3'... Gm5CCANTG Gm5C...5'

(No cleavage effect)

EcoKI: May overlap - effect not determined

EcoBI: May overlap - effect not determined

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BstEII
Content And Storage	Keep container tightly closed
Cut Site	G.GTNACC
Form	Liquid

Components	25 to 50% Water, >50% Glycerin
pH	7.4
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity	2000 U
Product Type	BstEII

Description