OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Supplier: Thermo Fisher Scientific BP80241

Catalog No. BP8024-1

Quantity

Description

5'...A T^C G A T...3'
3'...T A G C^T A...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 8.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycero

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with ClaI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of ClaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Note:

ClaI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain.

Compatible Ends: Bsp119I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, TaqI, XmiI

Methylation Effects:

Dam: May overlap - blocked

3'...TAGC Tm6AG...5'

(Cleavage is blocked)

Dcm: Never overlaps - no effect

CpG: Completely overlaps - blocked

(Cleavage is blocked)

EcoKI: Never overlaps - no effect

EcoBI: May overlap - effect not determined

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Cfr9I
Content And Storage	Keep container tightly closed
Cut Site	AT.CGAT
Form	Liquid

Components	>50% Water, 25 to 50% Glycerin
pH	7.4
Storage Buffer	10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
Quantity	600 U
Product Type	ClaI (Bsu15I)

Description