OPTIZYME™ HinfI, Fisher BioReagents™

Supplier: Thermo Fisher Scientific BP80511

Catalog No. BP8051-1

Quantity

Description

5'...G^A N T C...3'
3'...C T N A^G...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂, 100mM KCl and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with HinfI.

Compatible Ends: G^AWTC – PfeI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: May overlap - cleavage impaired

3'...CTNA Gm5C...5'

(Cleavage is impaired)

3'... Gm5CTNA Gm5C...5'

(Cleavage is impaired)

EcoKI: Never overlaps - no effect

EcoBI: May overlap - blocked

3'...AC TNAG(N)₅m6ACGA...5'

(Cleavage is blocked)

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity	2000 U
Product Type	HinfI

Components	25 to 50% Water, >50% Glycerin
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Content And Storage	Keep container tightly closed
Cut Site	G.ANTC
Form	Liquid

Description