OPTIZYME™ HpaII, Fisher BioReagents™

Supplier: Thermo Fisher Scientific BP80321

Catalog No. BP8032-1

Quantity

Description

5'...C^C G G...3'
3'...G G C^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with HpaII.

Compatible Ends: Bsp119I, Bsu15I, Hin1I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Completely overlaps - blocked

(Cleavage is blocked)

EcoKI: Never overlaps - no effect

EcoBI: Never overlaps - no effect

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity	1000 U
Product Type	HpaII

Components	25 to 50% Water, >50% Glycerin
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
Content And Storage	Keep container tightly closed
Cut Site	C.CGG
Form	Liquid

Description