OPTIZYME™ NdeI, Fisher BioReagents™

Supplier: Thermo Fisher Scientific BP80201

Catalog No. BP8020-1

Quantity

Description

5'...C A^T A T G...3'
3'...G T A T^A C...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 100mM NaCl and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with NdeI.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of NdeI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: Csp6I, FspBI, Tru1I, VspI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: May overlap - no effect

3'...AC T(N)₄GTATm6ACGA...5'

(No effect on cleavage)

Specifications

Concentration	10 U/μL
pH	7.4
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity	1500 U
Product Type	NdeI

Components	25 to 50% Water, >50% Glycerin
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Nde I
Content And Storage	Keep container tightly closed
Cut Site	CA.TATG
Form	Liquid

Description