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OPTIZYME™ SphI (PaeI), Fisher BioReagents™
Supplier: Thermo Fisher Scientific BP80291
Description
5'...G C A T G^C...3'
3'...C^G T A C G...5'
Supplied with: 10X OPTIZYME Buffer 1
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 1: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 100%
- Buffer 2: 50 - 100%
- Buffer 3: 0 - 20%
- Buffer 4: 50 - 100%
- Buffer 5: 0 - 20%
- 10mM potassium-phosphate (pH 7.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/ml BSA and 50% (v/v) glycerol
- More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with SphI.
- Digestion of Agarose-embedded DNA:
- A minimum of 5 units of SphI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
- Compatible Ends: Hin1II, XceI
- Methylation Effects:
- Dam: Never overlaps - no effect
- Dcm: Never overlaps - no effect
- CpG: May overlap - no effect
- 5'...m5C GCATGm5C G...3'
- 3'... Gm5CGTAC Gm5C...5'
- (No effect on cleavage)
- EcoKI: Never overlaps - no effect
- EcoBI: May overlap - blocked
- 5'...TGm6A(N)5GCA TGCT...3'
- 3'...AC T(N)5CGTm6ACGA...5'
- (Cleavage is blocked)
Specifications
| 10 U/μL | |
| 37°C | |
| >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with SphI | |
| 500 U | |
| SphI |
| 10X OPTIZYME™ Buffer 1 | |
| 7.4 | |
| 10mM Potassium Phosphate (pH 7 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.5mg/mL BSA, 50% Glycerol | |
| GCATG.C | |
| Liquid |