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Promega pCI and pCI-neo Mammalian Expression Vectors 
Contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in mammalian cells.
Supplier: Promega E1841
Description
pCI Mammalian Expression Vector
- Designed to promote constitutive expression of cloned DNA inserts in mammalian cells
- Contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region†
- Can be used for both transient and stable expression of genes
- Note: For stable expression, the pCI Vector must also be transfected with an expression vector containing a selectable gene for mammalian cells
- Similar to pCI Mammalian Expression Vector
- Also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells
- Can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418
- Can also be used to synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication
Specifications
| Neo | |
| TE Buffer (pH 8) | |
| 1 mg/mL | |
| pCI-neo vector |
| CMV | |
| -30°C to -10°C | |
| 20 μg | |
| Promote constitutive expression of cloned DNA inserts in Mammalian cells, can also be used to synthesize transcripts in-vitro using the T7 RNA Polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication |