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Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Supplier: Thermo Scientific™ 23030
Description
Thermo Scientific™ Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.
Highlights:
- Forms covalent bonds with sulfhydryl groups at pH 6.5-7.5
- Reaction can be monitored by measuring the decrease in absorbance at 300nm
Specifications
| Thiol | |
| Unreactive Group | |
| Pierce™ | |
| Medium (10 to 30 AÌ) | |
| 25 g | |
| NEM |
| Upon receipt store at -20°C. | |
| Blocking Groups | |
| Maleimide | |
| Solid | |
| Water |
For Research Use Only. Not for use in diagnostic procedures.