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Thermo Scientific™ Pierce™ BCA Protein Assay Reagent B
Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.
Supplier: Thermo Scientific™ 23224
Description
Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.
Additional Information and Online Ordering
Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
Highlights:
- Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
- Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
- Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
- Moderately fast – much easier and four times faster than the classical Lowry method
- High linearity – linear working range for BSA equals 20 to 2000μg/mL
- Sensitive – detect down to 5μg/mL with the enhanced protocol
BCA Protein Assay Applications:
- Studying protein:protein interactions
- Measuring column fractions after affinity chromatography
- Estimating percent recovery of membrane proteins from cell extracts
- High-throughput screening of fusion proteins
Specifications
| Store at room temperature. | |
| BCA Assay | |
| Pierce™ | |
| Not Target-Specific | |
| Spectrophotometer, Microplate Reader |
| Pierce BCA Protein Assay Reagent B | |
| Colorimetric | |
| Protein Quantitation Assay | |
| Solution-based Detection, Absorbance | |
| 25 mL |
For Research Use Only. Not for use in diagnostic procedures.