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Thermo Scientific™ Metal Enhanced DAB Substrate Kit
Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.
Supplier: Thermo Scientific™ 34065
Description
Thermo Scientific™ Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.
The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The near-black signal (precipitate) is easy to photograph.Highlights:
Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB
Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent
Six-hour stability – working solution is stable for more than six hours at room temperature
Includes:
Metal Enhanced DAB (10X), 25mL; Stable Peroxide Buffer, 250mL
Specifications
| Chromogenic, Colorimetric | |
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| HRP (Horseradish Peroxidase) Substrate | |
| Immunodetection, IHC (Immunohistochemistry) |
| 275 mL of 1X working solution | |
| DAB (Enhanced) | |
| Pierce™ | |
| DAB Substrate Kit |
For Research Use Only. Not for use in diagnostic procedures.