Thermo Scientific™ Pierce™ Streptavidin Poly-HRP

Description

Thermo Scientific™ Pierce Streptavidin Poly-HRP Conjugate is streptavidin biotin-binding protein that is conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

This poly-HRP conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. Pierce Streptavidin Poly-HRP is purified to remove unconjugated streptavidin molecules that reduce signal intensity by competing for binding sites with horseradish peroxidase (HRP)-conjugates molecules. In addition, the conjugate is devoid of HRP monomers that can cause background signal. The Pierce Streptavidin Poly-HRP is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.

Highlights:

• High sensitivity – detect low-abundant targets (low picogram-femtogram range) with high signal-to-noise ratios

• Robust – consistent manufacturing and purification to eliminate low- and un-conjugated molecules for minimal background and high sensitivity

• Flexible – compatible with chromogenic, fluorogenic and chemiluminescent substrates

• Versatile – compatible with ELISA, Western blotting and immunohistochemistry (IHC)

• Easy to use – can be directly substituted into immunoassays and other detection assays

• Convenient – ready-to-use stabilized liquid format stored at 4°C

• Cost effective – requires less conjugate per assay and standard HRP conjugates