Promega Calf Intestinal Alkaline Phosphatase (CIAP) Buffer Pack

A complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 PNK and [gamma-32P]ATP.

Supplier: Promega M1833

Catalog No. PR-M1833

Quantity

Description

Phosphorylates RNA, ssDNA and dsDNA Ends with a Purchased Radiolabeled Nucleotide
System includes enzymes, buffers and control DNA standards for measuring reaction efficiencies (except radionucleotides)
Flexible labeling works with [Î³-32P]ATP, [Î³-33P]ATP or [Î³-35S]ATP

Specifications

Components	0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
For Use With (Application)	Used with Alkaline Phosphatase Enzyme to preventing religation of linearized cloning vehicle DNA by removing Phosphate groups from both 5' terminals
Quantity	3 x 500 μL
Form	Liquid

Description