Promega Dual-Luciferase™ Reporter (DLR™) Assay System Passive Lysis 5X Buffer 

Description

• Rapid Lysis of Cultured Mammalian Cells
• No need for scraping adherent cells or performing freeze-thaw cycles
• Minimal coelenterazine autoluminescence
• Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Dual-Luciferase(R) Reporter Assay System (Cat.# E1910)

• U.S. Pat. Nos. 7,078,181, 7,108,996 and 7,118,878 have been issued to Promega Corporation for a method for increasing luminescence assay sensitivity. Other patents are pending.
• This product does not convey a license to use recombinant italics (begin)Renillaitalics (end) luciferase under U.S. Pat. No. 5,418,155 and related patents. Promega sells licensed italics (begin)Renillaitalics (end) luciferase vectors, which may be used in conjunction with this product.
• The method of recombinant expression of italics (begin)Coleopteraitalics (end) luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
• U.S. Pat. No. 5,744,320, Australian Pat. No. 721172, European Pat. No. 0 833 939, Canadian Pat. No. 2,221,522, Japanese Pat. No. 3601606 and Korean Pat. No. 10-438880 have been issued to Promega Corporation for quenching reagents and assays for enzyme-mediated luminescence.
• U.S. Pat. Nos. 5,641,641, 5,650,289 and 5,814,471, Australian Pat. No. 649289, European Pat. No. 0 553 234 and Japanese Pat. No. 3171595 have been issued to Promega Corporation for a beetle luciferase assay method, which affords greater light output with improved kinetics as compared to the conventional assay.
• Certain applications of this product may require licenses from others.