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Promega Flexi™ Cloning System
The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.
Supplier: Promega C8640
Includes: 10X Flexi Enzyme Blend (Sgf I and Pme I), 2X Flexi Ligase Buffer, 5X Flexi Digest Buffer, Nuclease-free water, T4 DNA ligase, Wizard™ SV Gel and PCR Clean-Up System (C8640 only)
Description
- Directional Cloning of Protein-Coding Sequences
- Choose from a variety of initial applications (e.g., bacterial protein, mammalian or cell-free protein expression) and then transfer the insert as required
- Efficient insert transfer means direct use of recombinant clones with minimal time for screening background colonies
- Adaptable to high-throughput formats for large screening projects
1L5671, L5681: Licensed under U.S. Pat. No. 5,910,628.
2For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
3This product or portions thereof is manufactured under license from Amrad Corporation Limited. For noncommercial research use only. All other uses require a license from Amrad Corporation Limited, Unit 6, 663 Victoria Street, Abbotsford, Victoria 3067, Australia, under U.S. Patent 5,654,176; Australian Patent 607511, Canadian Patent 1338903 and other issued patents.
4U.S. Patent 5,391,487.
5Licensed under U.S. Patent 5,945,288.
6The pF indicates that the vector is a Flexi vector. The letter after pF indicates the position of any expression tags (N = an N-terminal expression tag). The number associated with the vector specifies the type of expression and application. The letters A and K designate the bacterial drug selection for the vector (A = ampicillin and K = kanamycin).
Specifications
100ng/μL | |
Inducible expression of HQ- and GST-tagged fusion Proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter | |
20 | |
Fluid |
COMPONENTS AT DIFFERENT TEMPS | |
10X Flexi Enzyme blend (Sgf I and Pme I), 2X flexi Ligase Buffer, 5X flexi Digest Buffer, Nuclease-free Water, T4 DNA Ligase, Wizard™ SV Gel and PCR clean up system (C8640 only) | |
8 |