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Promega T4 DNA Ligase 
For ligation of DNA ends.
Supplier: Promega M1794
Description
- Joins Two DNA Strands with Cohesive or Blunt Ends
- Use with 5′, 3′ or blunt-ended dsDNA (e.g., inserts and vectors)
- Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
- Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
Specifications
| 10 to 20 U/μL | |
| 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol | |
| Recombinant E. coli strain | |
| T4 DNA Ligase | |
| Liquid |
| Colorless | |
| >90% | |
| Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts) | |
| -30°C to -10°C | |
| 500 U | |
| 68 |