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Promega T4 RNA Ligase 
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.
Supplier: Promega M1051
Description
- Joins ssRNA or ssDNA onto the 5′-Phosphoryl End of ssRNA or ssDNA
- Adds [5′-32P] nucleoside 3′,5′-bis (phosphate) onto single-stranded RNA
- Can be inactivated by heating at 65°C for 15 minutes
- Supplied at a concentration of 10u/μL
Specifications
| 10 U/μL | |
| 10X Reaction Buffer: 500mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 50mM DTT, 10mM ATP | |
| 7.5 | |
| 10mM Tris (pH 7.5 at 25°C), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1% Tween™20, 50% Glycerol | |
| Recombinant E. coli strain | |
| T4 RNA Ligase | |
| Liquid |
| Colorless | |
| >90% | |
| Ligation of single-stranded Nucleic Acids, 3' end-labeling of RNA, circularization of RNA and DNA Oligonucleotides, cloning full length cDNA | |
| -30°C to -10°C | |
| 500 U | |
| 43.5 |