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Cytiva Protein A and Protein G Mag Sepharose™
Simplify enrichment of target proteins by immunoprecipitation or pull-down applications
$94.97 - $235.83
Specifications
| Matrix | Paramagnetic spherical, highly cross linked agarose particles |
|---|---|
| Particle Size | 37 to 100 μm |
| Content And Storage | 4°C to 8°C |
| Catalog Number | Mfr. No. | Quantity | Binding Capacity | Ligand | Bead Type | Price | Quantity | ||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Binding Capacity | Ligand | Bead Type | Price | Quantity | ||
|
45-002-161
|
Cytiva
28944006 |
500 μL | 8 to 17mg Human IgG/mL | Native Protein A | Protein A Mag Sepharose |
N/A
|
|
||
|
45-002-160
|
Cytiva
28944008 |
500 μL | 13 to 22mg Human IgG/mL | Protein G | Protein G Mag Sepharose |
N/A
|
|
||
|
45-002-173
|
Cytiva
28951379 |
4 x 500 μL | 13 to 22mg Human IgG/mL | Protein G | Protein G Mag Sepharose |
N/A
|
|
||
|
45-002-174
|
Cytiva
28951378 |
4 x 500 μL | 8 to 17mg Human IgG/mL | Native Protein A | Protein A Mag Sepharose |
N/A
|
|
||
Description
Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.
There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.
Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
- Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed
Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: Native protein A; Native protein G
Binding capacity Protein A: 8-17mg human IgG/mL gel
Binding capacity Protein G: 13-22mg human IgG/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% ethanol
Storage temperature: 4°C to 8°C
Specifications
| Paramagnetic spherical, highly cross linked agarose particles | |
| 37 to 100 μm | |
| 4°C to 8°C |