Thermo Scientific™ Remel™ Kovacs Indole Reagent

Description

Determine the ability of an organism to split indole from tryptophan molecule with Thermo Scientific™ Remel™ Kovac's Indole Reagent.Previously xylene was used to detect small amounts of indole produced from breakdown of tryptophan. Kovac's used amyl alcohol in place of ethyl alcohol to bypass extraction procedure involved in the Ehrlich-Boehme reagent1. Gadebusch and Gabriel reported that substitution of isoamyl alcohol resulted in greater stability of the Kovacs' reagent2.

Kovac’s Indole Reagent is used to determine the ability of microorganisms, primarily Enterobacteriaceae, to split indole from the tryptophan molecule. Intracellular enzymes collectively called tryptophanases help in the production of indole by hydrolytic activity against the amino acid tryptophan. Indole combines with dimethylaminobenzaldehyde to form a red compound. This is a condensation reaction3.Ready for use: No further preparation is required.Easy to interpret: A pink-red ring between broth and solvent iindicates a positive testKovac’s Indole Reagent contains amyl alcohol (CAS 71-41-0), p-dimethylaminobenzaldehyde (CAS 100-10-7), hydrochloric acid (concentrated) (CAS 7647-01-0). This reagent has been tested using quality control procedures and found to be acceptable. This product is for in vitro diagnostic use and should be used by properly trained individuals.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.