Thermo Scientific™ Remel™ Nitrate Reagent B For AFB

Description

Differentially identify Mycobacterium spp. with their ability to utilize nitrate using Thermo Scientific™ Remel™ Nitrate Reagent B For AFB. Virtanen found that mycobacteria differ in their ability to reduce nitrate by means of nitrate reductase based on the age of the culture, temperature, enzyme inhibitors, and hydrogen ion concentration1. The nitrate reduction procedure is recommended by Kent and Kubica in A Guide for the Level III Laboratory2.

Mycobacteria, being a slow growing bacteria, needs a faster detection in the specimen samples for rapid diagnosis and treatment of Tuberculosis. Nitrate reduction is the most sensitive test used for detecting the ability of Mycobacteria to reduce nitrate. The presence of the enzyme nitrate reductase is an important differential characteristic in identifying different species of Mycobacteria.Ready to Use: Convenience of a prepared reagent.Ease of Differentiation: Positive test indicates red color development after addition of reagents; and no color development after addition of zinc dust. Negative test indicates no color development after addition of reagents and red color development after addition of zinc dust.Rapid Detection: within 30-60 seconds.The end products of nitrate reduction are many and depend upon the bacterial species. The most important end product is molecular nitrogen by way of nitrite reduction which is indicated by the presence of a catabolic end product or the absence of nitrate in the medium. The development of a red color after the addition of three reagents: hydrochloric acid; sulphanilic acid and N-naphthylethylenediamine indicates that the nitrate has bene reduced to nitrite. The red color reaction is due to the formation of a diazonium compound. When confirming a negative test with zinc dust, the reduction of the diazonium salt by zinc in the presence of acetic acid produces a colored compound, arylhydrazine.Used in conjunction with Nitrate Reagent A for AFB, Part No. R21243. For use with Nitrate Substrate Broth, Part No. R0615 Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.