Thermo Scientific™ PathoDx™ Chlamydia Culture Confirmation Kit

Description

Detect and identify Chlamydia by immunofluorescence technique after growth in tissue culture with Thermo Scientific™ Remel™ PathoDx™ Chlamydia Culture Confirmation Kit. Chlamydiae are the most common etiologic agents in sexually transmitted diseases¹. The genus Chlamydia is divided into three species: C. trachomatis, C. psittaci, and C. pneumoniae, which share a common lipopolysaccharide antigen². C. trachomatis is responsible for serious and often asymptomatic infections in humans³. It is currently recognized as the cause of cervicitis and pelvic inflammatory disease (PID) in female^4,5,6.

The Chlamydia Culture Confirmation Kit uses fluorescein-conjugated murine monoclonal antibodies to stain inclusions and/or elementary bodies in cell monolayers 48 hours after inoculation of a clinical specimen. The fluorescein-labeled monoclonal antibodies recognize both the major outer membrane protein (MOMP) of all C. trachomatis serovars and the genus-specific lipopolysaccharide (LPS) of Chlamydia.

When the antibody conjugate is added to a cover slip containing ethanol-fixed tissue culture cells, the reagent binds specifically to any chlamydial inclusions present in the cells. A washing step removes unbound antibody conjugate. When the stained coverslip is viewed under a fluorescence microscope, large bright apple-green inclusions within infected cells are observed against a red counterstained background of cells.

• Ready-to-use – Liquid reagents
• Convenient – Color coded dropper bottles
• Easy-to-interpret – Fixed, stained cultures that are positive for chlamydiae contain inclusion bodies that fluoresce bright green, against a cell monolayer counter-stained dark red. The presence of one inclusion body is considered positive.

The Chlamydia Culture Confirmation Kit contains Chlamydia Confirmation Reagent and Mounting Fluid (R62230). Chlamydia Confirmation Reagent contains 5.5 mL of solution containing fluorescein-labeled purified murine monoclonal antibodies, Evans Blue counterstain, an inhibitor of nonspecific stain, and 0.098% sodium azide in a protein-stabilized buffer solution.

The Mounting Fluid provides 5.0 mL of mounting fluid, that contains of buffered glycerol, with 0.01% sodium azide as preservative.

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