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Cytiva Sephadex™ G50 Superfine Media
Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.
Supplier: Cytiva 17004101
Description
- For purposes of determining maximum flowrate, beads behave as rigid spheres according to Darcy's Law
- Working pH range 2 to 10
- Particle size range for wet beads 20 to 80μm
- Bed volume in distilled water 9 to 11mL/g
- Fractionation ranges: 5 x 102 to 1 x 104 for dextrans, 1.5 x 103 to 3 x 104 for globular proteins
Specifications
| 100 g | |
| 20 to 80 μm in wet form, 20 to 50 μm in dry form | |
| Sephadex G50 Media | |
| 5cm | |
| 3.93 in. | |
| min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d. | |
| Cross-linked Dextran | |
| 4 to 30°C |
| < Darcy´s Law | |
| 9 to 11mg/g | |
| Solid Bead | |
| Purification of DNA from small molecules by Gel filtration (higher efficiency with shorter diffusion distances) | |
| 10cm | |
| 2 to 10 | |
| DNA |