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Cytiva Sepharose™ Fast Flow Cation Exchange Media
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
Supplier: Cytiva 17072901
Description
Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.
- Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
- Composed of crosslinked 6% agarose beads
- Reproducible
- Part of Sepharose Fast Flow ion exchange platform
- High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
- Available in range of pre-packed process development tools
SP Sepharose:
- Ligand: Sulphopropyl
- Ion Exchanger Type: Strong cation exchanger
- Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
- Binding capacity: 70mg ribonuclease A/mL medium
- Ligand: Carboxymethyl
- Ion Exchanger Type: Weak cation exchanger
- Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
- Binding capacity: 50mg ribonuclease A/mL medium
Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals
Specifications
| 300 mL | |
| Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol | |
| Strong CatIon Exchanger | |
| 70 mg Ribonuclease A/mL mediμm | |
| 400 to 700 cm/Hoμr | |
| 100 kPa | |
| 4 to 13 | |
| Sulphopropyl |
| For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals | |
| 4°C to 30°C | |
| 0.18 to 0.25 mM Hl+/mL | |
| SP Sepharose Fast Flow Media | |
| 45 to 165 μm | |
| 6% Cross-Linked Agarose | |
| 20% Ethanol, 0.2 M Sodium Acetate | |
| 300 mL |