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MilliporeSigma™ TAT-CRE Recombinase Protein
Catalyzes site specific recombination event between two loxP DNA sites. MilliporeSigma™; TAT-CRE Recombinase is a recombinant cell-permeant fusion cre-recombinase protein consisting of TAT sequence, a nuclear localization sequence (NLS).
Supplier: MilliporeSigma™ SCR508
Description
- Consists of basic protein translocation peptide derived from HIV-TAT (TAT), a nuclear localization sequence (NLS), the Cre protein and an N-terminal histidine tag (H6) for efficient purification of the protein from E. coli
- Effectively excises STEMCCA viral transgenes from both Human and Mouse IPS cells
- Purity: single band around 41kDa with greater than 70% protein purity on SDS-PAGE gel
- Functional activity: Mediates recombination of LoxP-modified alleles in HEK293T- Cre reporter cell line
- Endotoxin levels: less than 1 EU/ug protein
- Mycoplasma negative
Specifications
Cell Culture, Stem Cell Culture | |
E.coli | |
<1 EU/ug protein |
1500 U | |
Stable for 3 months from date of receipt when stored at -20°C or -80°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C. | |
TAT-CRE Recombinase Protein |