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Gibco™ Viral Production Cells 2.0 
Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.
Supplier: Gibco™ A51218
Description
- Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line
- They are a core component of the AAV-MAX Helper-Free AAV Production System
- The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium
- Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
- Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
- Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
Features of the Viral Production Cells 2.0 include:
- Clonal, 293F-derived, high-producer cell line
- Absence of SV40 large T antigen
- Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
- Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
- Robust scalability and passage stability
Specifications
| Frozen | |
| 6 cryovials, store in vapor phase liquid nitrogen | |
| Kidney (Embryonic) | |
| Frozen | |
| 6 vials | |
| Gibco Viral Production Medium | |
| AAV-MAX | |
| 1 mL | |
| Human |
| 1e7 cells/mL | |
| Viral Production Cells 2.0 | |
| Suspension Cell Culture | |
| Human | |
| AAV Production | |
| 1 x 107 cells | |
| Viral Production Cells | |
| dry ice for domestic shipment; liquid nitrogen for international shipment |
For Research Use Only. Not for use in diagnostic procedures.