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Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL) DFS Item

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Supplier:  Invitrogen™ AM2548

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Catalog No. AM2548


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Description

Description

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Proteinase K remains active:
• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g., in the presence of SDS or urea)
• In the presence of metal chelating agents (e.g., EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Specifications

Specifications

20 mg/mL
Store at -20°C.
Shipped at ambient temperature.
5 x 1.25 mL
Ambion™
T. album
Dry Ice
Proteinase K
Liquid
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For Research Use Only. Not for use in diagnostic procedures.