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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Supplier: Cytiva 17524701
Description
- Prepacked with Ni Sepharose™ High Performance medium
- Negligible nickel ion leakage
- Pressure Max: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Manual operation with syringe, pump or chromatography system
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: 3 to 12
1mL columns
- Bed Dimensions: 7 x 25mm
- Flow velocity: 4mL/min.
5mL columns
- Bed Dimensions: 16 x 25mm
- Flow velocity: 5mL/min.
Specifications
| 1 mL | |
| 7 mm | |
| 4 mL/min. (1 mL), 5 mL/min. (5 mL) | |
| 3 to 12 | |
| 4°C to 30°C | |
| 5 x 1 mL |
| 0.2 in. | |
| HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. | |
| Polypropylene | |
| 6% Highly Cross-linked Agarose | |
| 5 bar (0.5 MPa, 70 psi) | |
| Pre-packed Column |