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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
$84.30 - $1053.23
Specifications
| Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
|---|---|
| Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
| Material | Polypropylene |
| pH Range | 3 to 12 |
| Support Type | 6% Highly Cross-linked Agarose |
| Catalog Number | Mfr. No. | Quantity | Diameter (Metric) Inner | Price | Quantity | ||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Diameter (Metric) Inner | Price | Quantity | ||||
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45-000-323
|
Cytiva
17524701 |
5 x 1 mL | 7 mm |
Pack of 5 Each for $316.93
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45-000-324
|
Cytiva
17524801 |
5 mL | 16 mm |
Each for $268.55
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45-000-325
|
Cytiva
17524802 |
5 x 5 mL | 16 mm |
Pack of 5 Each for $1,053.23
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|
45-000-513
|
Cytiva
29051021 |
1 mL | 7 mm |
Each for $84.30
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Description
- Prepacked with Ni Sepharose™ High Performance medium
- Negligible nickel ion leakage
- Pressure Max: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Manual operation with syringe, pump or chromatography system
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: 3 to 12
1mL columns
- Bed Dimensions: 7 x 25mm
- Flow velocity: 4mL/min.
5mL columns
- Bed Dimensions: 16 x 25mm
- Flow velocity: 5mL/min.
Specifications
| HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. | |
| Polypropylene | |
| 6% Highly Cross-linked Agarose | |
| 5 bar (0.5 MPa, 70 psi) |
| 4 mL/min. (1 mL), 5 mL/min. (5 mL) | |
| 3 to 12 | |
| 4°C to 30°C | |
| Pre-packed Column |