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Cytiva Single Cell GenomiPhi™ DNA Amplification Kit
Amplify whole genome DNA from as little as a single cell. Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
Supplier: Cytiva 29108107
Description
- For whole genome amplification of genomic DNA from as little as a single cell
- Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
- Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
- Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
- Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
- Reaction time: 2 to 4 hours
- Product average size: >10kb
For SNP arrays, Next Gen Sequencing, aCGH
Recommended StorageStore the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C
Specifications
| Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. | |
| illustra™ Single Cell GenomiPhi™ DNA Amplification Kit | |
| Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL | |
| illustra Single Cell GenomiPhi DNA Amplification Kit | |
| 2 to 4 hours |
| Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference. | |
| Genomic DNA Amplification | |
| 25 | |
| >10kb |