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Invitrogen™ Proteinase K, recombinant DFS Item

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

Supplier:  Invitrogen™ 25530031

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Catalog No. 25530031


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Description

Description

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

  • Over a wide pH range–optimal activity between 6.5 and 9.5
  • Under denaturing conditions–e.g., in the presence of SDS or urea
  • In the presence of metal chelating agents–e.g., EDTA
  • At comparatively high temperatures–optimum digestion temperature is 65°C
  • Protein Filter: P

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Specifications

Specifications

>40 U/mg
Approved for shipment at Room Temperature or on Wet Ice
Proteinase K
One tube Proteinase K
Store in refrigerator (2–8°C).
1 g
Powder
SDS
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For Research Use Only. Not for use in diagnostic procedures.