Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

Supplier: Invitrogen™ SS04

Catalog No. SS04

Quantity

Description

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution
As TMB substrate solution reacts with horseradish peroxidase, it produces a pronounced blue color
At the end of TMB incubation, a volume of ELISA Stop Solution equal to the volume of TMB substrate solution is added to the wells to terminate the horseradish peroxidase reaction
Addition of Stop Solution results in the rapid formation of a yellow end product
The optical density of this yellow end product is measured at 450 nm
The yellow end product is stable for sixty minutes after addition of Stop Solution, optical density should be measured within this time period
Store at 2°C to 8°C

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Description