OPTIZYME™ BglII, Fisher BioReagents™

Supplier: Thermo Fisher Scientific BP80145

Catalog No. BP8014-5

Quantity

Description

5'...A^G A T C T...3'
3'...T C T A G^A...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl₂, 100mM NaCl and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 8.2 at 25°C), 200 mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with BglII.

Digestion of Agarose-embedded DNA:

A minimum of 5 units of BglII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends: BamHI, BclI, Bsp143I, MboI, PsuI

Methylation Effects:

Dam: Completely overlaps - no effect

(No effect on cleavage)

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: May overlap - cleavage impaired

3'...AC TCTAGA(N)₃m6ACGA...5'

(Cleavage is impaired)

Specifications

Concentration	10 U/μL
Incubator Temperature	37°C
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
Content And Storage	Keep container tightly closed
Cut Site	A.GATCT
Form	Liquid

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
pH	7.4
Storage Buffer	10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
Quantity	2500 U
Product Type	BglII

Description