Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media

Used for capture and intermediate purification requiring low to medium hydrophobicity

Supplier: Cytiva 17097310

Catalog No. 45-002-940

Quantity

Description

Base matrix derivatized via uncharged, chemically-stable ether linkages
Two levels of substitution help find optimal selectivity and binding capacity for given application
Hydrophilic nature of the base matrix ensures low levels of non-specific binding
Hydrophobic interaction chromatography (HIC) media composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages
Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development
Degree of substitution for low sub HIC media is ∽25μmol phenyl/mL gel
Degree of substitution for high sub HIC media is ∽40μmol phenyl/mL gel

Specifications

Quantity	25 mL
Stability	Stable in commonly used aqueous Buffers: 1 M NaOH, 3 M Ammonium Sulphate, 30% Isopropanol, 70% Ethanol, 10% Ethylene Glycol, 0.5% SDS, 6 M Guanidine-Hydrochloride, 8 M Urea
Product Type	Phenyl Sepharose 6 Fast Flow Media (high sub)
Particle Size	45 to 165 μm
pH Range	3 to 13
Ligand Type	Phenyl

For Use With (Application)	For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity
Storage Requirements	4°C to 30°C
Flow Rate	250 to 400 cm/Hoμr
Matrix	6% Cross-Linked Agarose
Storage Buffer	20% Ethanol

Description