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Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

$336.80 - $806.06

Specifications

Detection Location In-Gel Detection
Detection Method Colorimetric
Label or Dye Silver
Product Type Stain Kit
Product Line Pierce™
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
PI24612
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Thermo Scientific™
24612
1 L
Each for $336.80
 
FER24612X3
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Thermo Scientific™
24612X3
3 x 1 L
Each for $806.06
 
Description

Description

Thermo Scientific™ Pierce Silver Stain Kit is a rapid, ultra-sensitive and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results with many 1D and 2D gel types for both first-time and experienced users.

The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Features:
Sensitivity—detect proteins at 0.25 ng
Protocol—gel staining can be performed within 5 min to 20 hrs, depending on desired sensitivity
Gel-compatibility—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing, and other downstream procedures that depend on destaining and protein recovery

Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions, but the concentration is low enough that irreversible protein crosslinking is minimized.

Specifications

Specifications

In-Gel Detection
Silver
Pierce™
Colorimetric
Stain Kit
Protein
SDS
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For Research Use Only. Not for use in diagnostic procedures.