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Promega Alkaline Phosphatase, Calf Intestinal 
Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.
$100.87 - $102.77
Specifications
| Color | Colorless |
|---|---|
| Components | 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine |
| pH | 8 |
| For Use With (Application) | Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase |
| Storage Buffer | 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol |
Description
- Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs
- Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL
- Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications
Specifications
| Colorless | |
| 8 | |
| 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol | |
| Calf intestinal mucosa | |
| Alkaline Phosphatase |
| 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine | |
| Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase | |
| -30°C to -10°C | |
| 1000 U | |
| Liquid |