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Promega pGEM™-T and pGEM™-T Easy Vector Systems 
Convenient systems for the routine subcloning of PCR products
Supplier: Promega A1360
Includes: Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only)
Description
The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid.
- 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide
- f1 Origin of Replication allows the preparation of single-stranded DNA
pGEM-T Vector Systems
- Multiple cloning site is flanked by recognition sites for the restriction enzyme BstZ I Single-enzyme digestion allows release of the insert Double digestion may also be used to release the insert from the vector
- Recognition sites for BstZ I, EcoR I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion
Specifications
| SP6, T7 | |
| -30°C to -10°C | |
| pGEM-T easy vector I |
| BstZI, NotI and EcoRI | |
| 20 Reactions | |
| For the routine subcloning of PCR products |