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Promega pGEM™-T and pGEM™-T Easy Vector Systems DFS Item

Convenient systems for the routine subcloning of PCR products

$235.59 - $475.12

Specifications

Promoter SP6, T7
Quantity 20 Reactions
For Use With (Application) For the routine subcloning of PCR products
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Includes: Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only)

Products 4
Catalog Number Mfr. No. Restriction Site Content And Storage Vector Price Quantity  
Catalog Number Mfr. No. Restriction Site Content And Storage Vector Price Quantity  
PR-A1360 DFS Item
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Promega
A1360
BstZI, NotI and EcoRI -30°C to -10°C pGEM-T easy vector I
Each for $284.28
 
PR-A1380 DFS Item
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Promega
A1380
BstZI, NotI and EcoRI <-65°C pGEM-T easy vector II
Each for $475.12
 
PR-A3610 DFS Item
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Promega
A3610
BstZI <-65°C pGEM-T vector II
Each for $427.73
 
PR-A3600 DFS Item
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Promega
A3600
BstZI -30°C to -10°C pGEM-T vector I
Each for $235.59
 
Description

Description

The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid.

  • 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide
  • f1 Origin of Replication allows the preparation of single-stranded DNA

pGEM-T Vector Systems

  • Multiple cloning site is flanked by recognition sites for the restriction enzyme BstZ I Single-enzyme digestion allows release of the insert
  • Double digestion may also be used to release the insert from the vector
pGEM-T Easy Vector Systems
  • Recognition sites for BstZ I, EcoR I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion

Specifications

Specifications

SP6, T7
20 Reactions
For the routine subcloning of PCR products
SDS
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