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Promega Wizard™ Plus SV Minipreps DNA Purification System DFS Item

A silica membrane-based system for simple, rapid isolation of plasmid DNA from 1-10ml E. coli cultures. The miniprep procedure can be completed in 45 minutes or less.

Supplier:  Promega A1465

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Catalog No. PRA1465


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Includes:
  • DNA purification kit: Cell resuspension solution, cell lysis solution, neutralization solution, column wash solution, alkaline protease solution, nuclease-free water, miniprep spin columns, collection tubes with or without vacuum adapters
  • DNA purification start-up kit (110V): Wizard™ Plus SV Minipreps DNA Purification System, Welch™ vacuum pump, Vac-Man™ laboratory vacuum manifold, vacuum adapters

Description

Description

  • 1-20μg High-Quality Plasmid DNA from 1-10mL Cultures
  • Process up to 20 minipreps in 45 minutes
  • Choice of spin or vacuum purification formats
  • No phenol extractions or precipitations required

†Vacuum protocol requires use of a vacuum manifold.

Specifications

Specifications

15°C to 30°C
Produces high yield plasmid DNA of consistent quality from bacterial cultures
1000 Preps
1 to 20μg
Spin or Vacuum
4 × 250 Wizard SV minicolumns, 4 × 250 Collection tubes, 4 × 100mL Column wash solution, 4 × 3mL Alkaline Protease solution, 4 × 100mL Neutralization solution, 4 × 75mL Cell resuspension solution, 4 × 75mL Cell Lysis solution, 2 × 25mL Nuclease-free Water
DNA Purification System
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U.S. Patent 5,981,235, Australian Patent 729932.