Cytiva Thermo Sequenase DNA Polymerase

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

Supplier: Cytiva E79000Y

Catalog No. 09928126

Quantity

Description

Uniform and easy-to-read band patterns
Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed

Specifications

Content And Storage	-20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
Quantity	1,000 Units
No. of Reactions	32 units/μL
Source	E. coli clones that overproduce the protein

Description	Thermo Sequenase DNA Polymerase
For Use With (Application)	Sequencing, Genotyping
Includes	Thermo Sequenase with Thermoplasma Acidophilum Inorganic Pyrophosphatase (TAP), Dilution Buffer, and Reaction Buffer

Description