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Cytiva Thermo Sequenase DNA Polymerase
Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates
$1691.39 - $10995.56
Specifications
| Content And Storage | -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol |
|---|---|
| Description | Thermo Sequenase DNA Polymerase |
| For Use With (Application) | Sequencing, Genotyping |
| No. of Reactions | 32 units/μL |
| Source | E. coli clones that overproduce the protein |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
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09928126
|
Cytiva
E79000Y |
1,000 Units |
Each for $1,691.39
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09928127
|
Cytiva
E79000Z |
10,000 Units |
N/A
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N/A | |||||
| Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance. | |||||||||
Description
- Uniform and easy-to-read band patterns
- Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
- Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
- ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
- Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
- An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
- Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed
Specifications
| -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol | |
| Sequencing, Genotyping | |
| E. coli clones that overproduce the protein |
| Thermo Sequenase DNA Polymerase | |
| 32 units/μL |