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Cytiva His SpinTrap™ Spin Columns
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.
$691.44 - $692.71
Specifications
| Description | His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) |
|---|---|
| Support Type | 6% Highly Cross-linked Agarose |
| Content And Storage | 4°C to 30°C |
| Type | Spin Column Kit |
| Catalog Number | Mfr. No. | Type | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Type | Price | Quantity | |||||
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45-001-427
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Cytiva
28401353 |
Spin Column Kit |
Pack of 50 Each for $691.44
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45-002-017
|
Cytiva
28932171 |
Spin Column Kit |
Each for $692.71
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Description
- Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
- One run in standard microcentrifuge takes approximatedly 10 min.
- Yields up to 750μg purified protein per column
- Allows direct purification of both clarified and unclarifed cell lysates
- Also useful for screening large numbers of small-scale bacterial lysates
- Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
Medium Characteristics:
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Stable (long-term) in the pH range 3-12
- Average particle size: 34μm
Order Info
Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)
Specifications
| His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) | |
| 6% Highly Cross-linked Agarose | |
| 4°C to 30°C | |
| Spin Column Kit |