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MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates
Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns
Supplier: MilliporeSigma™ WBLUF0500
Description
Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.
- Consistent results with less pipetting error
The stabilizers added to Luminata substrates:
- Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
- Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
- Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation
Three different sensitivities for a range of detection needs:
- Immobilon Classico premixed reagent for everyday detection needs
- Immobilon Crescendo premixed reagent for detection in the picogram range
- Immobilon Forte premixed reagent for detection in the femtogram range
Specifications
| HRP Substrate | |
| 500 mL | |
| 18°C to 24°C |
| Dot/Slot Blot | |
| ∼400 fg |