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Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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1 – 15 of 33 results
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Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

2

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Shipping Condition Wet Ice
Substrate Horseradish Peroxidase
Sensitivity Low-femtogram to high-attogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence
3

Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

4

MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Product Type HRP Substrate
For Use With (Application) Dot/Slot Blot
5

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate Horseradish Peroxidase
Sensitivity Mid-femtogram
Form Liquid
Product Type Substrate
Signal Duration 24 hr.
Product Line SuperSignal
Detection Method Chemiluminescence
6

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

7

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

8

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

9

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Contains SuperSignal™ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.

10

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

11

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Content And Storage 2°C to 8°C
Kit Contents Detection reagent 1 and 2
Product Type ECL Western Blot Substrate
13

MilliporeSigma™ Luminata Crescendo ELISA HRP Substrate

Used on immunoblots as chemiluminescent substrate

Content And Storage 2°C to 8°C
14

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Substrate Horseradish Peroxidase
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form Liquid
Product Type Substrate
Signal Duration 1 to 2 hr.
Product Line Pierce
Detection Method Chemiluminescence
15

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.